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Health Management

Fish in Assam

Health Management

HEALTH MANAGEMENT IN AQUACULTURE
Introduction:

Disease is one of the major problems in carp culture system in Assam. Fish suffer ill health from a variety of reasons like Environment (water), Fish (host) and pathogens, which are all inter-related. Any fragile imbalance between the fish and environment causes the disease outbreak. Interaction of host, pathogen and environment affects the disease state of the fish. So, improving water quality, adopting scientific methods of fish culture and maintaining eco-friendly environment will reduce the disease outbreak.

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To avoid the disease problem one has to take care in maintaining a healthy environment during the culture period. One of the best methods to avoid this problem is to follow the scientific method of fish culture, because fish health management starts from the beginning of site selection of pond till the harvesting of fish is a multidisciplinary approach through which we can maintain a good health of fish. The good health management programmes includes such as

  1. Proper selection of pond site.
  2. Good pond design construction.
  3. Preparation of pond before stocking of fish.
  4. Stocking of quality fish seed.
  5. Right stocking density.
  6. Maintaining stable phytoplankton growth through fertilization.
  7. Water quality parameters management.
  8. Proper feeding with balanced diet.
  9. Avoid physical injury while netting and stocking.
  10. Avoid frequent netting in winter season.
  11. Avoid keeping much organic load in the pond bottom.
  12. Random monitoring of health condition of the fish during culture period.
  13. Keeping record of events (disease outbreak) happening in the pond.
Main root causes of disease outbreak are:
  1. When the pond water become polluted.
  2. High stocking density, resulting overcrowding of fish.
  3. When cultured fishes are deprived of sufficient amount of nutritious feed.
  4. When the fishes are attacked by pathogens (parasites, bacteria, fungi and virus) in the ambient.
  5. High organic load in the pond bottom.
  6. Introduction of exotic fishes (new arrival).
Behavioural signs of healthy fish:
  1. Clean body with shining.
  2. Fins are kept in erected condition.
  3. Bright body colour.
  4. Faster movement.
  5. Fins and skins remain in intact conditions.
  6. Gills are reddish in colour.
  7. Show defensive struggle.
  8. Movement of eyes is faster.
Behavioural changes of unhealthy fish:
  1. Irregular swimming movements.
  2. Swimming out of water.
  3. Rubbing the body against any rough surfaces.
  4. Surfacing on water with shoaling behaviour.
  5. Gasping air.
  6. Refusal of feed.
  7. Excessive mucus secretion on the body surface.
  8. Eyes are shrunken.
  9. Blackish body colour.
  10. Swelling of abdomen.
  11. Cyst formation on the body surface.
  12. Fins are erected.
  13. Very lethargic in movement.
  14. Maxillary extension.
  15. Head becomes larger.
Treatment strategies to follow:

For any deciding treatments mainly depends on the following factors:

  1. Knowledge about the disease development process.
  2. Knowledge about the pathogen.
  3. Knowledge about the environment.
  4. Knowledge about the host.
Essential to know the existence of the pathogen for proper treatment to be followed
  1. Whether the pathogen existence in soil?
  2. Whether the pathogen existence in water?
  3. Whether the pathogen existence in the surface of the body?
  4. Whether the pathogen existence in gut (endoparasite)?
  5. Whether the pathogen is a systemic?
General precautions and remedies are to be followed:
  1. Stocking density of fish should be at optimum level.
  2. Before stocking fish seed, ensure that pond is free from insects and predators.
  3. Lime and fertilizers should be used as recommended, doses as per POP.
  4. Provide optimum quantity of feed.
  5. Maintain optimum level of PH and DO2 primarily.
  6. Do not allow thick growth of plankton.
  7. Prevent entry of fish eating birds.
  8. Dead and infected fish should be removed immediately.
  9. Removal of molluscs from the pond bottom by netting or dewatering.
  10. Maintain higher Alkalinity during winter season.
  11. Reduce more handling during transportation.
  12. Reduce netting during winter season.
  13. While stocking fish seed into the rearing pond bath the fishes with 1-2 ppm with KMnO4 (Potash) for 1 minute, then release into the pond.
  14. Avoid entry of sewage/drainage water into the pond.
Different types of fish diseases:
  1. Parasitic fish diseases (Ecto and Endoparasites).
  2. Bacterial fish diseases.
  3. Fungal fish diseases.
  4. Viral fish diseases.
  5. Nutritional related fish diseases.
  6. Environmental mediated fish diseases.
Source of infection in cultured fish:
  1. Entry of infected fish.
  2. Carrier fish (no clinical signs).
  3. Contaminated eggs in hatchery operation.
  4. Infected water supply.
  5. Contaminated feeds.
  6. Use of contaminated nets, boats, and equipment.
Treatment of fish diseases:

Several kinds of treatments are available depending on the intensity and type of diseases, a few disease treatments for some common diseases and their treatments are given below:

Table 1: Common fish diseases and their treatment
Diseases Symptoms Treatment
A. PROTOZOAN DIESEASE
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White Spot Disease
b) Trichodiniasis Causative agent: Trichodina spp. Greyish blue veil like coating over the body surface and gills, colour of gills turn pale, sluggish and shows asphyxia. Smear from gills shows saucer-shape with fringe cilia under microscope Bath: Nacl 2-3% solution till the fishes are stressed at KMnO4 @ 4mg/L (OR) Formalin @ 25 mg/L in pond.
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Tricodina infection (Source Internet)

c) Costiasis (Blue slim disease) Causative agent: Costia necatrix Bluish patches on the skin surface. More affected areas are reddened and become haemorrhagic. Short bath: Nacl @ 25mg/L for 10-15 minutes. (OR) KMnO4 @ 1 mg/L of water for 5-10 minutes.
d) Whirling disease Causative agent: Myxobolus cerebralis Whirling movement of the fish, malformation of the vertebral column, cranium etc. Difficult to control, removed the sick fish from the pond.
e) White scale spot disease Causative agent: Myxobolus mrigalae Specially found in mrigala and rohu where scales and body surface are covered with whitish cysts located on the body surface and scale. While in rohu situated superficially as well as inside the scale also. Bath: Nacl @ 3-5% solution. (OR) Mohua oil cake and lime can be applied in the pond
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Myxobolus infection
Myxobolus infection
a) Dactylogyrosis and Gyrodactylosis Causative agent: Dactylogyrus sp. and Gyrodactylus spp. In dactylogyrosis, the colour of gills fade and excessive mucus secretion occurs. While in gyrodactylosis, there is fading of the normal body colour and small spots of blood on the body surface may be seen with naked eye or with the help of magnifying lens. Bath: NaCl @ 3-5% solution for 15 minutes (OR) Formalin @ 100 mg/L for 5-10 minutes or @ 25 mg/L in pond water. (OR) KMnO4 @ 4-5 ppm in pond water.
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Dactylogyrus infection (Gill Fluke)
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Gyrodactylus infection (Skin Fluke)
b) Black spot disease Causative agent: Diplostomum spp. or Isoparorchis hypselobagri Black ovoid patches are visible on the body surface and these are pigmented overlaying cysts of the metacercarial larvae. Number of cysts may be few to hundreds. Removal of resident molluscan population (1st intermediate host) and the aquatic birds (final host) around it.
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Black spot infection
c) Ligulosis Causative agent: Ligula intestinalis Affected fishes are dark in colour; abdomen is distended, because of the presence of large number of pleurocercoid larvae. Control methods are limited, entry should be restricted of the definitive host, the ichthyophagous birds.
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Ligula intestinalis infection
d) Acanthocephalan disease Causative agent: Acanthogyrus acanthogyrus No visible external symptoms to determine the infection. Parasites inhabit in elementary tract of fish with the help of hooks present. Site of attachment is swollen and reddish in colour. Proper treatment methods have not developed
C. CRUSTACEAN DISEASE
a) Argulosis (Fish louse disease) Causative agent: Argulus spp. Affected fish become restless with erratic swimming movement, ulcer formation at attachment site. Adult parasite is oval, flat and transparent to whitish in colour with two conspicuous black eye spot. It can be seen clearly with naked eye. Bath: Nacl @ 3-5% solution till the fishes are stressed. (OR) Gamaxine @ 1 ppm in pond water. Short bath: KMnO4 @ 10 ppm for 30 minutes or @ 4 ppm in pond
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Argulus Infection

b) Ergasilosis Causative agent: Ergasilus spp. Infestation occurs in gills, buccal cavity, operculum and fins. They look like white bodies less than 2 mm long. Fishes are surfacing, lethargy and restless. Bath: Nacl @ 3-5 % solution for 15 minutes. (OR) KMnO4 @ 4ppm in pond water.
Bath: Nacl @ 3-5 % solution for 15 minutes. (OR) KMnO4 @ 4ppm in pond water. Fish become restless, parasites penetrates into the scale and keeps hanging egg sac, clearly visible to the naked eye and attachment site shows ulceration. Dip treatment: DDT @ 10 ppm for 30 seconds. (OR) Short bath: NaCl @ 3-5% solution. (OR) KMnO4 @ 4 ppm in pond water.
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Lernea Infection

d) Other parasites Leeches Attach form, blood sucking with its proboscis. Attachment areas show ulcer formation. Quicklime @ 1 gm/2 litres of water in pond. (OR) Nacl @ 3-5 % solution
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Leech Infection
D. BACTERIAL DISEASE
a) Eye disease of catla Causative agent: Aeromonas liquefaciens The eyes look reddish due to vascularisation and later on become opaque pockets thereby making scale loose. Abdomen of the fish gets distended. KMnO4 @ 5 ppm in pond water or 1 ppm in pond as a prophylactic
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Eye Infection
b) Ulcer disease Causative agent: Aeromonas liquefaciens and Pseudomonas flurorescens The eyes looks reddish due to vascularisation and later on become opaque pockets thereby making scale loose. Abdomen of fish gets distended. KMnO4 @ 5 ppm in pond water and Feed: Sulphadiazine @ 100 mg/kg of feed or Terramycin @75-80 mg/kg body weight of fish 10-12 days.
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Ulcer
c) Fin and Tail rot disease Causative agent: Aeromonas liquefaceins and Pseudomonas flurorescens Disintegration of fins and tail of fish with whitish margin as thread. Disintegration of fins and tail of fish with whitish margin as thread.
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Fin and Tail rot
d) Dropsy Causative agent: Aeromonas and Psedomonas species Accumulation of fluid in epidermis and body cavity leading to bulging of belly. KMnO4 @ 5 ppm in pond water.
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Dropsy
e) Vibriosis Causative agent: Vibrio spp. Small red spots or petechiae on the throat, under the opercula and in the ventral and cardiac region and just immediately anterior to the pelvic fins. Later reddish coloration of the dermis and muscle tissue. Bath: Vaccine may be used or Hemisulphate 20 ppm for 30 minutes.
E. FUNGAL DISEASE
a) Saprolegniasis (Cotton wool disease) Causative agent: Saprolegnia parasitica Pathogen grows over any necrotic tissues of the imparting a cotton wool like growth or even on eggs. Colour depends on the particles that are trapped inside. KMnO4 @ 160 ppm with affected fish till stressed for 5 days or 100 ppm in swab treatment for 7 days. (OR) Malachite green @ 1-2 ppm for 30 minutes. (OR) Formalin @ 20 ppm in pond water.
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Saprolegnia infection
b) Branchiomycosis (gill rot disease) Causative agent: Branchiomyces demigrans Gill lose their normal colour and turn yellowish brown, gradually portions of the gills may degenerate. Affected fish; surface and gasping air. Bath: Liming @ 50 kg/ha and NaCl @ 3-4 % solution for bath treatment
F. OTHER DISEASES
a) Deformities: Possible cause: Hereditary conditions Lack of fins, swim bladder, debility and certain skeletal deformities occurs as hereditary conditions, the origin of which seems to be due to an inherited disorder of vitamin D metabolism. Prevention: Avoid from stocking of such origin source or farm
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Bone deformities
b) Pin head disease: Possible causes: Nutritional requirement Head becomes bigger compared to the body growth and posterior end become slender. Prevention: improve feed quality with nutritionally complete diet requirement and reduce the stocking density.
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Pin Head Disease
G. ENVIRONMENTAL MEDIATED DISEASES
a) Asphyxiation Causes: Oxygen depletion Fishes gaps for air at surface of water particularly during the period preceding dawn and immediately after. It also occurs during cloudy weather or after a short heavy rainfall. Measures: Spray water over the surface of the pond by using water pump or pond water should be agitated manually to facilitate oxygen uptake.
b) Acidosis and Alkalosis Cause: Variation of PH PH < 4.8: Acidosis PH> 10: Alkalosis A brown coating on the gills consisting of necrotic epithelium. The coating becomes thicker as exposure goes on, but eventually breaks up and sloughs off. The skin assumes a muddy appearance and shed layers of thick mucus. Redness, eating away of gills and fraying of fins is the symptoms of alkalosis. Measures: PH adjustment with application of lime.
c) Gas bubble disease Causes: Super saturation of oxygen and Nitrogen in water Tiny bubbles at the periphery of eye, near the scales on the gills etc. The abdomen is swollen. Measures: Stop application of fertilizers immediately and add freshwater to the ponds.
d) Algal toxicosis disease C.A: Microcystis and Anabaena spp. Surfacing of fish with erratic movement and cause many mortality in many cases due to clogging of gills resulting in respiratory distress. CuSO4 @ 0.5 ppm in pond (OR) Cow dung @ 200 kg/ha is sprinkled over the surface. Some surface area of pond is covered with water hyacinth to prevent sunlight penetration.
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Harmful Algal Bloom (HAB)
Epizootic Ulcerative Syndrome (EUS)

It is a freshwater and brackish water fish disease. It is not reported in marine water so far. Occurring in tropical warm water fish mainly in the Indo-pacific region. This disease was first reported in 1972 in Australia, particularly in the Queens land lake. In the year, 1988 it was reported in India in the month of May in Tripura in canals, rivers, ponds and paddy fields, which caused mass mortality of fish.

Definition of EUS: EUS is a seasonal epizootic condition of freshwater and estuarine warm water fish of complex infectious etiology characterised by the presence of invasive Aphanomyces infection and necrotising ulcerative lesion typically leading to granulomatous response.

Species affected: All freshwater species and most prone species are the murrels.

Possible factors in outbreak of EUS:
  1. Drop in temperature in freshwater systems.
  2. Sudden drop in salinity < 1.5 ppt.
  3. Sudden rains and flooding in freshwater systems.

Causative Agent: Aphanomyces invadans

Clinical signs:
  • Gradually Red blood blotches are visible on the body surface and later turns into ulcers exposing the bones.
  • Surfacing and gasping of air by the affected fishes.
  • Fishes die off in large quantities within short period of time.
Control Measures:

1. Quick lime (Cao) @ 100-600 kg/ha depending on the alkalinity of water.

2. KMnO4 @ 1-10 ppm

i) Pond treatment @ 5-10 ppm OR

ii) Bath treatment @ 1-6 ppm for 5 minutes.

3. Bleaching powder @ 1 mg/L OR 5-10 kg/ha/m in pond water.

4. CIFRI package:

i) Quick lime (CaO) @ 50 kg/ha prior to outbreak of disease which areas are prone to disease. (Prophylactic)

ii) Quick lime (CaO) @ 100 kg/ha during outbreak and after 7 days add 1 ppm bleaching powder.

5. Nacl @ 3-4% dip treatment of affected fish for 30 second.

6. CIFAX application: 1 litre/ha/m depth of water initially and second dose after 15 days of interval.

7. Lime and Turmeric powder: Use mixture of lime and turmeric powder 9:1 kg/bigha in pond water.

8. Antibiotics: Oxytetracycline, Erythromycin @ 60-100 mg/kg of fish for 7 days in feed.


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EUS infection
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